Scientist Maps DNA Of New Citrus Greening Strain

Feed The LeavesA University of Florida researcher has mapped the DNA genome of a new strain of citrus greening that could further threaten Florida’s beleaguered citrus industry. Knowing the genetic makeup of the various strains is critical to finding a cure.

Dean Gabriel, a plant bacteriology specialist with UF/IFAS, helped sequence and map the genome of the most prevalent form of the disease in Florida, and now he and colleagues have done the same for a new strain of the disease discovered in Brazil.

There is no cure for either strain, although researchers believe that knowing the genetic makeup of the disease is critical to finding one. Gabriel said by having that “roadmap” of the bacteria genome, they will be certain there are no surprises in the Brazilian species, which has now been found in Texas.  In addition, the mapping should help guide them to improvements in control methods and toward more usable genes and treatments.

“What the genome does, it lets you know everything that the organism has and doesn’t have in its artillery for offense and defense — and it lets you design a strategy to control it,” Gabriel said.

Researchers often liken having the genetic sequence for an organism to having its list of parts.

“Having all the genetic information is like having a detailed roadmap of the organism,” said Jackie Burns, director of the UF/IFAS Citrus Research and Education Center in Lake Alfred.

Gabriel’s team’s work will be outlined in a research paper that will be published in February in the journal Molecular Plant-Microbe Interaction. The genome map is already available online, at GenBank.

To map the bacteria’s DNA genome, Gabriel’s Brazilian colleagues first diced up and crushed tissue from the veins of infected citrus trees where the organism was most highly concentrated.  They used chemicals to extract DNA and purified it. The team had to separate the tree DNA from that of the bacterium.

DNA comprises four nucleotides, which fall into an order to encode genes specific to an organism. Gabriel likened it to examining beads on a necklace: The beads come in only four colors, and the color sequence determines each gene — in this case, the DNA greening “necklace” held 1,195,201 beads, or 1,044 genes.

To obtain the nucleotide sequence from the purified bacterial DNA, they used state-of-the-art sequencing machines at the UF Interdisciplinary Center for Biotechnology Research.

The research was funded by the Citrus Research and Development Foundation Inc., an affiliate UF/IFAS.

Source: UF/IFAS

Topics: , , ,

Leave a Reply

Citrus Stories
blue orchard bee
CitrusPlight Of Pollinators Presenting Sticky Situation
June 24, 2015
Growers, beekeepers, and the ag-chem industry urged to work together to protect pollinators and plants. Read More
dripping orange
CitrusFlorida Legislative Session Bittersweet For Citrus
June 23, 2015
Gov. Rick Scott OKs almost $20 million to fight HLB; vetoes nearly $4 million in other related initiatives. Read More
CitrusCalifornia High Heat Warning Issued
June 23, 2015
The California Division of Occupational Safety and Health (Cal/OSHA) is advising all employers to protect their outdoor workers from the Read More
Close-up of Sugar Belle LB 8-9
Varieties & RootstocksSounds Like Chime Is Right For Sugar Belle LB 8-9
June 22, 2015
Well-vetted variety is finding its sweet spot amid today’s HLB-influenced production practices. Read More
non-gmo label leafy greens
CitrusResearchers Ready To Testify Before Congress About GMOs
June 22, 2015
UF/IFAS grad students seeking to field questions relating to the GMO regulatory processes, food labeling, and product safety. Read More
2015 Florida Citrus Industry Annual Conference crowd
CitrusFlorida Citrus Growers’ Resolve Put To The Test
June 22, 2015
During annual conference, industry leaders call for an ‘all in’ approach to survive current crisis. Read More
Insect & Disease UpdateAsian Citrus Psyllid Quarantine Expanded Again In California
June 22, 2015
Following an Asian citrus psyllid (ACP) find in San Luis Obispo County in May the California Department of Food and Read More
The Latest
CitrusIndustry Stakeholders Rip Report Linking Citrus Consump…
July 1, 2015
Associations react swiftly; call out flaws in study released by the Journal of Clinical Oncology. Read More
money down the drain
CitrusGovernor’s Budget Veto Likely To Shutter UF/IFAS …
June 25, 2015
A $180,000 increase, plus another $720,000 in funding for program gone in the stroke of a pen. Read More
blue orchard bee
CitrusPlight Of Pollinators Presenting Sticky Situation
June 24, 2015
Growers, beekeepers, and the ag-chem industry urged to work together to protect pollinators and plants. Read More
dripping orange
CitrusFlorida Legislative Session Bittersweet For Citrus
June 23, 2015
Gov. Rick Scott OKs almost $20 million to fight HLB; vetoes nearly $4 million in other related initiatives. Read More
CitrusCalifornia High Heat Warning Issued
June 23, 2015
The California Division of Occupational Safety and Health (Cal/OSHA) is advising all employers to protect their outdoor workers from the Read More
non-gmo label leafy greens
CitrusResearchers Ready To Testify Before Congress About GMOs
June 22, 2015
UF/IFAS grad students seeking to field questions relating to the GMO regulatory processes, food labeling, and product safety. Read More
2015 Florida Citrus Industry Annual Conference crowd
CitrusFlorida Citrus Growers’ Resolve Put To The Test
June 22, 2015
During annual conference, industry leaders call for an ‘all in’ approach to survive current crisis. Read More
CitrusPollinator Protection Dependent On Pure Partnerships [O…
June 18, 2015
Florida bee BMPs program has not only improved cooperation between growers and beekeepers, but also shows that voluntary efforts work. Read More
UF/IFAS certificate of farm labor management
CitrusFlorida Growers Working Toward Safety And Equality
June 17, 2015
New Certificate Program educates farm supervisors on best practices. Read More
CitrusPresident Obama Allocates $110M For Drought-Relief
June 16, 2015
On Friday, President Obama announced the investment of $110 million to support workers, farmers, and rural communities suffering from drought Read More
Lake Howell in Casselberry, FL
CitrusFarm Bureau Offers Detailed Analysis Of Waters Of The U…
June 15, 2015
Rule will give EPA “sweeping powers” to regulate land use, according to the American Farm Bureau Federation. Read More
Florida BMPs grower meeting
CitrusFlorida BMPs Program Racks Up More Successes
June 15, 2015
The Florida legislature passed the 1999 Florida Watershed Restoration Act that gives the Florida Department of Agriculture and Consumer Services Read More
secrets can do lots of damage
CitrusConfidentiality Counterproductive For Today’s Far…
June 15, 2015
No longer is transparency an anomaly among the grower community, it's become necessary to survive and succeed. Read More
squeezing an orange
CitrusFlorida Citrus Season Grinding Toward Sour Finish
June 10, 2015
USDA’s updated estimate keeps orange count in check at historically low total. Read More
Charlotte's Web-medical marijuana in greenhouse
CitrusFlorida Still Playing Medical Marijuana Waiting Game
June 9, 2015
Growers and potential patients hold their breath while next steps are taken to spin Charlotte’s Web. Read More
CitrusParamount Farms Renamed As Part Of The Wonderful Compan…
June 9, 2015
Roll Global officially changed its name to The Wonderful Company recently. This strategic move will align the company’s long-standing passion Read More
Guava Fruit Fly specimen
CitrusLos Angeles County Under Quarantine Following Guava Fru…
June 9, 2015
A portion of Los Angeles County has been placed under quarantine for the guava fruit fly (GFF) following the detection Read More
motorized conveyor belt for citrus juice processing
CitrusFlorida Juice Plant Put On Auction Block
June 8, 2015
Financial troubles catch up with Ft. Pierce manufacturer and bottler as feds seize all assets. Read More