Scientist Maps DNA Of New Citrus Greening Strain

Feed The LeavesA University of Florida researcher has mapped the DNA genome of a new strain of citrus greening that could further threaten Florida’s beleaguered citrus industry. Knowing the genetic makeup of the various strains is critical to finding a cure.

Dean Gabriel, a plant bacteriology specialist with UF/IFAS, helped sequence and map the genome of the most prevalent form of the disease in Florida, and now he and colleagues have done the same for a new strain of the disease discovered in Brazil.

There is no cure for either strain, although researchers believe that knowing the genetic makeup of the disease is critical to finding one. Gabriel said by having that “roadmap” of the bacteria genome, they will be certain there are no surprises in the Brazilian species, which has now been found in Texas.  In addition, the mapping should help guide them to improvements in control methods and toward more usable genes and treatments.

“What the genome does, it lets you know everything that the organism has and doesn’t have in its artillery for offense and defense — and it lets you design a strategy to control it,” Gabriel said.

Researchers often liken having the genetic sequence for an organism to having its list of parts.

“Having all the genetic information is like having a detailed roadmap of the organism,” said Jackie Burns, director of the UF/IFAS Citrus Research and Education Center in Lake Alfred.

Gabriel’s team’s work will be outlined in a research paper that will be published in February in the journal Molecular Plant-Microbe Interaction. The genome map is already available online, at GenBank.

To map the bacteria’s DNA genome, Gabriel’s Brazilian colleagues first diced up and crushed tissue from the veins of infected citrus trees where the organism was most highly concentrated.  They used chemicals to extract DNA and purified it. The team had to separate the tree DNA from that of the bacterium.

DNA comprises four nucleotides, which fall into an order to encode genes specific to an organism. Gabriel likened it to examining beads on a necklace: The beads come in only four colors, and the color sequence determines each gene — in this case, the DNA greening “necklace” held 1,195,201 beads, or 1,044 genes.

To obtain the nucleotide sequence from the purified bacterial DNA, they used state-of-the-art sequencing machines at the UF Interdisciplinary Center for Biotechnology Research.

The research was funded by the Citrus Research and Development Foundation Inc., an affiliate UF/IFAS.

Source: UF/IFAS

Topics: , , ,

Leave a Reply

Citrus Stories
photo illustration showing confusion
Citrus
June 24, 2016
Is It EPA’s Intention To Help Or Harm Farmers? [Opinion]
A common theme I see in complaints about the government agency is it appears to be taking action more and more based on ideology and public opinion rather than sound science. Read More
produce family shopping grocery
Citrus
June 23, 2016
Survey: Consumers Hungry For More Info On GMOs
University of Florida researchers find learning opportunity among poll results regarding genetically modified foods. Read More
2015 FFVA Annual Convention crowd
Citrus
June 23, 2016
Florida Fruit & Vegetable Association Convention To Cover Critical Issues
Mike Allen, a Founder of Politico and its Chief White House Correspondent, slated as keynote speaker for association's 73rd annual gathering. Read More
UAV in flight
Citrus
June 22, 2016
Heads Up: FAA Finalizes Rules For Small Drones
Regulations will create new opportunities for business and government entities to use unmanned aircraft systems weighing less than 55 pounds. Read More
overflowing beaker in science lab
Citrus
June 21, 2016
Sound Science Struggling To Win Over Skeptics [Opinion]
We all want the truth. But can we handle it? Read More
Florida Citrus Industry Annual Conference 2016 crowd
Citrus
June 20, 2016
Florida Citrus Community Rallies Around Promise Of Bactericides, Biotechnology
Industry stakeholders holding out hope for solutions to HLB through antimicrobials and even transgenics. Read More
Precision Citrus LLC's shadeshouse construction site near Lake Wales, FL
Citrus
June 16, 2016
Florida Citrus Nurseries Seeking To Make It In The Shade
Undercover production plans and possibilities starting to take shape. Read More
The Latest
Citrus
June 28, 2016
Panama Canal Expands For New Trade Oppor…
First vessel passes through new, larger locks as country celebrates. Read More
Citrus
June 24, 2016
Is It EPA’s Intention To Help Or H…
A common theme I see in complaints about the government agency is it appears to be taking action more and more based on ideology and public opinion rather than sound science. Read More
Citrus
June 23, 2016
Survey: Consumers Hungry For More Info O…
University of Florida researchers find learning opportunity among poll results regarding genetically modified foods. Read More
Citrus
June 23, 2016
Florida Fruit & Vegetable Associatio…
Mike Allen, a Founder of Politico and its Chief White House Correspondent, slated as keynote speaker for association's 73rd annual gathering. Read More
Citrus
June 22, 2016
Heads Up: FAA Finalizes Rules For Small …
Regulations will create new opportunities for business and government entities to use unmanned aircraft systems weighing less than 55 pounds. Read More
Citrus
June 21, 2016
Sound Science Struggling To Win Over Ske…
We all want the truth. But can we handle it? Read More
Citrus
June 20, 2016
Florida Citrus Community Rallies Around …
Industry stakeholders holding out hope for solutions to HLB through antimicrobials and even transgenics. Read More
Citrus
June 16, 2016
Florida Citrus Nurseries Seeking To Make…
Undercover production plans and possibilities starting to take shape. Read More
Citrus
June 14, 2016
Monsanto Rejects Second Bid From Bayer
Wall Street Journal reports Monsanto seeks bid increase from Bayer. Read More
Citrus
June 10, 2016
Florida Orange Crop Estimate Climbs Agai…
For the fourth consecutive month, ground is gained in Valencias. Read More
Citrus
June 8, 2016
Study: New Yield Mapping Method Might He…
With the possibility of saving money on inputs and labor, UF/IFAS researchers focus to find immature fruit faster. Read More
Citrus
June 8, 2016
AgAmerica Lending Banks On Experience To…
Industry veteran hired to enhance growth of company’s farm loans portfolio throughout Florida and the Southeast U.S. Read More
Citrus
June 2, 2016
Storm Season Off To Extra-Soggy Start In…
No let-up for parts of the Sunshine State still trying to shake off from wettest dry season in nearly six decades. Read More
Citrus
June 1, 2016
Groups Make Push To Challenge DuPont, Do…
Letter from industry organizations say proposed merger could hinder innovation, raise prices, and reduce choices. Read More
Citrus
May 31, 2016
Cannabis Cultivation Quest Becoming Real…
Planning, preparations finally giving way to production and dispensing of ‘Charlotte’s Web.’ Read More
Citrus
May 25, 2016
Monsanto Says Bayer Bid ‘Financially Ina…
Proposal cited as undervalued, not able to address financial, regulatory risks. Read More
Citrus
May 25, 2016
NRCS Invests $4.3 Million To Combat Clim…
The Natural Resources Conservation Service in California is committing funds to help farmers and ranchers mitigate the effects of climate change and help to reduce greenhouse gas emissions. Read More
Citrus
May 24, 2016
Panama Canal Expansion To Bring Trade Op…
Agriculture Commissioner Adam Putnam says a confluence of events is putting the state's producers in a good spot to open new markets. Read More
[gravityform id="62" title="false" description="false"]