Scientist Maps DNA Of New Citrus Greening Strain

Feed The LeavesA University of Florida researcher has mapped the DNA genome of a new strain of citrus greening that could further threaten Florida’s beleaguered citrus industry. Knowing the genetic makeup of the various strains is critical to finding a cure.

Dean Gabriel, a plant bacteriology specialist with UF/IFAS, helped sequence and map the genome of the most prevalent form of the disease in Florida, and now he and colleagues have done the same for a new strain of the disease discovered in Brazil.

There is no cure for either strain, although researchers believe that knowing the genetic makeup of the disease is critical to finding one. Gabriel said by having that “roadmap” of the bacteria genome, they will be certain there are no surprises in the Brazilian species, which has now been found in Texas.  In addition, the mapping should help guide them to improvements in control methods and toward more usable genes and treatments.

“What the genome does, it lets you know everything that the organism has and doesn’t have in its artillery for offense and defense — and it lets you design a strategy to control it,” Gabriel said.

Researchers often liken having the genetic sequence for an organism to having its list of parts.

“Having all the genetic information is like having a detailed roadmap of the organism,” said Jackie Burns, director of the UF/IFAS Citrus Research and Education Center in Lake Alfred.

Gabriel’s team’s work will be outlined in a research paper that will be published in February in the journal Molecular Plant-Microbe Interaction. The genome map is already available online, at GenBank.

To map the bacteria’s DNA genome, Gabriel’s Brazilian colleagues first diced up and crushed tissue from the veins of infected citrus trees where the organism was most highly concentrated.  They used chemicals to extract DNA and purified it. The team had to separate the tree DNA from that of the bacterium.

DNA comprises four nucleotides, which fall into an order to encode genes specific to an organism. Gabriel likened it to examining beads on a necklace: The beads come in only four colors, and the color sequence determines each gene — in this case, the DNA greening “necklace” held 1,195,201 beads, or 1,044 genes.

To obtain the nucleotide sequence from the purified bacterial DNA, they used state-of-the-art sequencing machines at the UF Interdisciplinary Center for Biotechnology Research.

The research was funded by the Citrus Research and Development Foundation Inc., an affiliate UF/IFAS.

Source: UF/IFAS

Topics: , , ,

Leave a Reply

Citrus Stories
CitrusBiocontrols 2015: Exhibition Sneak Preview
February 25, 2015
We take a look inside the exhibit hall at the Biocontrols 2015 Conference & Tradeshow. Read More
CitrusNew App Helps Identify Insects, Diseases, And Nutrient Deficiencies
February 24, 2015
Spensa Technologies launches app that helps growers take control of pests and improve soil nutrients. Read More
CitrusPacked Political Agenda On Tap For Florida Farmers
February 23, 2015
The Florida Fruit & Vegetable Association’s board of directors are working on a number of key issues that will be front-burner throughout 2015. Read More
CitrusWest Coast Governors Call For End To Port Labor Impasse
February 19, 2015
U.S. Secretary of Labor Thomas Perez helps mediate discussions between dockworkers and port operators. Read More
CitrusCompanion Biological Fungicide Now OMRI Listed
February 19, 2015
The biological fungicide from Growth Products is now registered for use in organic production. Read More
Varieties & RootstocksExperimental Florida Mandarins Measure Up
February 19, 2015
Variety display day results show Florida citrus research and development is on the right track. Read More
Insect & Disease UpdateScientists Cue Up 3D Printing In Quest To Quash Citrus Greening
February 18, 2015
Multidimensional technology used to build “SmartTrap” for vector of the disease. Read More
The Latest
Citrus18 Must-Have Apps For GenNext Growers
March 1, 2015
We asked about your favorite mobile applications, and here's what you had to say. Read More
FNV logo
CitrusPMA Announces Funding For Major Produce Marketing Progr…
February 26, 2015
The produce industry is getting into brand marketing in a big way. The Produce Marketing Association (PMA) has announced the launch of a new promotional campaign - FNV - designed to take a page from the marketing strategies of big-money consumer brands like Nike and Apple. Read More
CitrusFDA, Federal Partners Develop New Method For Attributin…
February 25, 2015
Data was analyzed from nearly 1,000 outbreaks to assess which categories of foods were most responsible for making people sick. Read More
CitrusBiocontrols 2015: Exhibition Sneak Preview
February 25, 2015
We take a look inside the exhibit hall at the Biocontrols 2015 Conference & Tradeshow. Read More
CitrusNew App Helps Identify Insects, Diseases, And Nutrient …
February 24, 2015
Spensa Technologies launches app that helps growers take control of pests and improve soil nutrients. Read More
CitrusPacked Political Agenda On Tap For Florida Farmers
February 23, 2015
The Florida Fruit & Vegetable Association’s board of directors are working on a number of key issues that will be front-burner throughout 2015. Read More
CitrusWest Coast Governors Call For End To Port Labor Impasse
February 19, 2015
U.S. Secretary of Labor Thomas Perez helps mediate discussions between dockworkers and port operators. Read More
CitrusCompanion Biological Fungicide Now OMRI Listed
February 19, 2015
The biological fungicide from Growth Products is now registered for use in organic production. Read More
CitrusNew Desalination Technology May Be The Answer To Califo…
February 18, 2015
Researchers plan to address concerns with a process that will examine ways to turn seawater into drinkable water. Read More
David Holden
CitrusBiopesticides, Biostimulants Show Results In Fruits And…
February 18, 2015
From biostimulants in celery and avocado to biopesticides in citrus and strawberries, consultant David Holden conducts in-the-field research that attests to the effectiveness of biocontrols. Read More
CitrusMegadrought In The West Predicted By End Of The Century
February 18, 2015
Lowering greenhouse gases will reduce risk, scientists say. Read More
CitrusIRS Change Could Pay Off In More Citrus Plantings
February 17, 2015
Florida Citrus Mutual is pushing a proposal forward that will allow growers to directly write off putting new trees in the ground. Read More
CitrusFlorida Citrus Poised For A Comeback
February 12, 2015
About halfway through the 2014-2015 season, and the crop could go either way. Read More
CitrusTier 4 Regulations Pique Propane Interest
February 12, 2015
New rules are extending to non-road applications like irrigation engines. Read More
CitrusSearch Is On For The Next Miss Florida Citrus
February 11, 2015
Pre-Miss America pageant makes its return offering contestants a chance to represent the industry and further their education. Read More
CitrusIs The ‘Agripocalypse’ Nearing? [Opinion]
February 10, 2015
Recent report indicates time is not on agriculture's side. Action is needed now to keep future growers engaged. Read More
CitrusFlorida Citrus Crop Holding On
February 10, 2015
Updated USDA forecast shows no change in orange and grapefruit yield. Read More
CitrusAgriculture Among Top Paying College Degrees Of 2015
February 9, 2015
Report reveals good news for graduates looking to make some green in farming. Read More