Scientist Maps DNA Of New Citrus Greening Strain

Feed The LeavesA University of Florida researcher has mapped the DNA genome of a new strain of citrus greening that could further threaten Florida’s beleaguered citrus industry. Knowing the genetic makeup of the various strains is critical to finding a cure.

Dean Gabriel, a plant bacteriology specialist with UF/IFAS, helped sequence and map the genome of the most prevalent form of the disease in Florida, and now he and colleagues have done the same for a new strain of the disease discovered in Brazil.

There is no cure for either strain, although researchers believe that knowing the genetic makeup of the disease is critical to finding one. Gabriel said by having that “roadmap” of the bacteria genome, they will be certain there are no surprises in the Brazilian species, which has now been found in Texas.  In addition, the mapping should help guide them to improvements in control methods and toward more usable genes and treatments.

“What the genome does, it lets you know everything that the organism has and doesn’t have in its artillery for offense and defense — and it lets you design a strategy to control it,” Gabriel said.

Researchers often liken having the genetic sequence for an organism to having its list of parts.

“Having all the genetic information is like having a detailed roadmap of the organism,” said Jackie Burns, director of the UF/IFAS Citrus Research and Education Center in Lake Alfred.

Gabriel’s team’s work will be outlined in a research paper that will be published in February in the journal Molecular Plant-Microbe Interaction. The genome map is already available online, at GenBank.

To map the bacteria’s DNA genome, Gabriel’s Brazilian colleagues first diced up and crushed tissue from the veins of infected citrus trees where the organism was most highly concentrated.  They used chemicals to extract DNA and purified it. The team had to separate the tree DNA from that of the bacterium.

DNA comprises four nucleotides, which fall into an order to encode genes specific to an organism. Gabriel likened it to examining beads on a necklace: The beads come in only four colors, and the color sequence determines each gene — in this case, the DNA greening “necklace” held 1,195,201 beads, or 1,044 genes.

To obtain the nucleotide sequence from the purified bacterial DNA, they used state-of-the-art sequencing machines at the UF Interdisciplinary Center for Biotechnology Research.

The research was funded by the Citrus Research and Development Foundation Inc., an affiliate UF/IFAS.

Source: UF/IFAS

Topics: , , ,

Leave a Reply

Citrus Stories
Insect & Disease UpdateCalifornia’s Citrus Growers Face More Challenges Than Huanglongbing
August 27, 2015
As a kid growing up we all remember the arrival of Barnum & Bailey’s circus. The ads, the posters, the Read More
Virginia Tech campus in Blacksburg
CitrusCounting Down The Top U.S. Colleges For Natural Resources And Conservation
August 25, 2015
STEM-fueled area of study presents wide field of opportunity for future grads. Read More
Charlotte's Web-medical marijuana in greenhouse
CitrusMedical Marijuana Endeavor Anything But Pipe Dream For Florida Nurseries
August 20, 2015
While applicants await fate for one of the five available licenses to weave ‘Charlotte’s Web,’ best-case-scenarios play out. Read More
CitrusSouth Florida Farmers Feted For Water Quality Standards
August 20, 2015
Marking 20 straight years of success, producers help Everglades Agricultural Area exceed phosphorus reduction goals. Read More
old world bollworm climbing on vegetation
CitrusOld World Bollworm A Potential New Pain For Florida Farmers
August 20, 2015
Growers in the Sunshine State on high alert as serious pest is detected for the first time in the U.S. outside of port interceptions. Read More
CitrusUSDA To Pour $20 Million Into Frozen Orange Juice
August 20, 2015
Section 32 bonus purchase program money to help move mountain of surplus inventory. Read More
Sugar Belle brand LB 8-9 orange tree
Varieties & RootstocksPriority High On Producing Proprietary Citrus Varieties
August 19, 2015
Whether it’s industry acceptance or a business necessity, the number of operations registering to grow exclusive selections is increasing. Read More
The Latest
CitrusFederal Judge Blocks Clean Water Rule
August 28, 2015
  According to RISE (Responsible Industry For A Sound Environment), a federal judge in North Dakota has blocked implementation of Read More
Golden Rice
CitrusAnti-GMO-Fueled Threats Force Reallocation Of Biotech E…
August 27, 2015
University of Florida officials say plans are to re-allocate a donation intended to improve the public’s understanding of science after Read More
money
CitrusMonsanto Drops Current Bid For Syngenta Acquisition
August 27, 2015
Even with a revised proposal that sweetened the pot to $46.2 billion, financial expectations still fall flat. Read More
Virginia Tech campus in Blacksburg
CitrusCounting Down The Top U.S. Colleges For Natural Resourc…
August 25, 2015
STEM-fueled area of study presents wide field of opportunity for future grads. Read More
Charlotte's Web-medical marijuana in greenhouse
CitrusMedical Marijuana Endeavor Anything But Pipe Dream For …
August 20, 2015
While applicants await fate for one of the five available licenses to weave ‘Charlotte’s Web,’ best-case-scenarios play out. Read More
CitrusSouth Florida Farmers Feted For Water Quality Standards
August 20, 2015
Marking 20 straight years of success, producers help Everglades Agricultural Area exceed phosphorus reduction goals. Read More
old world bollworm climbing on vegetation
CitrusOld World Bollworm A Potential New Pain For Florida Far…
August 20, 2015
Growers in the Sunshine State on high alert as serious pest is detected for the first time in the U.S. outside of port interceptions. Read More
CitrusUSDA To Pour $20 Million Into Frozen Orange Juice
August 20, 2015
Section 32 bonus purchase program money to help move mountain of surplus inventory. Read More
CitrusMaps Show Impending Impact From WOTUS
August 19, 2015
Plots indicate expansion of EPA’s reach across Pennsylvania, Virginia, and Montana. Read More
branches covered in ice
CitrusWicked Winter Coming This Way Says Latest Farmer’s Alma…
August 17, 2015
Super El Niño-influenced weather patterns align to paint a less-than-pretty portrait. Read More
dripping orange
CitrusFeeling The Squeeze, Florida Citrus Taking Stock Of Fut…
August 12, 2015
A look back on the 2014-2015 citrus season reveals major economic questions, concerns. Read More
man trying to prevent from being boxed in by boxes
CitrusEconomic Progress A Threat To Box In Agriculture [Opini…
August 11, 2015
For growers, buffer zones, noise ordinances, nutrient runoff concerns, and sinkhole threats all are factors heightened with nearby neighbors. Instead of trying to beat them, why not join them? Read More
CitrusAlico Bucks Trend Related To Current Citrus Struggles
August 10, 2015
Despite the 2014-2015 season yielding smallest crop in nearly 50 years, Florida-based company boasts marked growth in harvest, sales. Read More
Keith Jones
CitrusBiopesticide Industry Alliance Names Keith Jones As New…
August 10, 2015
The Biopesticide Industry Alliance (BPIA) announced its selection of Keith Jones as its new executive director. Mr. Jones is an Read More
Immokalee< FL, Farm and Grove aerial view
CitrusFlorida Farmland Remains Hot Commodity
August 5, 2015
Recent multimillion-dollar deal involving Immokalee citrus grove is among several high-acreage, high-dollar transactions. Read More
CitrusResearchers Say Drones Will Play A Major Role In Farmin…
August 4, 2015
Texas A&M AgriLife researchers say the technology will offer growers precise information about their crops. Read More
woman smashing an alarm clock with a hammer
CitrusDon’t Become A Time Crunch Casualty
July 31, 2015
Finding the balance between work life and home life is easier said than done for GenNext Growers. Read More
money
CitrusBankrupt Florida Farm To Yield Plenty Of Land, Equipmen…
July 30, 2015
As many as seven different buyers could be part of this Chapter 11 proceeding. Read More