Scientist Maps DNA Of New Citrus Greening Strain

Feed The LeavesA University of Florida researcher has mapped the DNA genome of a new strain of citrus greening that could further threaten Florida’s beleaguered citrus industry. Knowing the genetic makeup of the various strains is critical to finding a cure.

Dean Gabriel, a plant bacteriology specialist with UF/IFAS, helped sequence and map the genome of the most prevalent form of the disease in Florida, and now he and colleagues have done the same for a new strain of the disease discovered in Brazil.

There is no cure for either strain, although researchers believe that knowing the genetic makeup of the disease is critical to finding one. Gabriel said by having that “roadmap” of the bacteria genome, they will be certain there are no surprises in the Brazilian species, which has now been found in Texas.  In addition, the mapping should help guide them to improvements in control methods and toward more usable genes and treatments.

“What the genome does, it lets you know everything that the organism has and doesn’t have in its artillery for offense and defense — and it lets you design a strategy to control it,” Gabriel said.

Researchers often liken having the genetic sequence for an organism to having its list of parts.

“Having all the genetic information is like having a detailed roadmap of the organism,” said Jackie Burns, director of the UF/IFAS Citrus Research and Education Center in Lake Alfred.

Gabriel’s team’s work will be outlined in a research paper that will be published in February in the journal Molecular Plant-Microbe Interaction. The genome map is already available online, at GenBank.

To map the bacteria’s DNA genome, Gabriel’s Brazilian colleagues first diced up and crushed tissue from the veins of infected citrus trees where the organism was most highly concentrated.  They used chemicals to extract DNA and purified it. The team had to separate the tree DNA from that of the bacterium.

DNA comprises four nucleotides, which fall into an order to encode genes specific to an organism. Gabriel likened it to examining beads on a necklace: The beads come in only four colors, and the color sequence determines each gene — in this case, the DNA greening “necklace” held 1,195,201 beads, or 1,044 genes.

To obtain the nucleotide sequence from the purified bacterial DNA, they used state-of-the-art sequencing machines at the UF Interdisciplinary Center for Biotechnology Research.

The research was funded by the Citrus Research and Development Foundation Inc., an affiliate UF/IFAS.

Source: UF/IFAS

Topics: , , ,

Leave a Reply

Citrus Stories
Citrus
July 22, 2017
Representative from Washington Proposes Amendment to H-2A Program
Move broadens use of H-2A to all of agriculture to include those with multiple crops and harvests. Read More
Insect & Disease Update
July 21, 2017
University of California Launches Website to Update Growers on Citrus Research
Easy-to-read format designed to give growers up-to-date information on huanglongbing and Asian citrus psyllid research. Read More
Hurricane Matthew satellite image as it brushed past Florida
Citrus
July 20, 2017
Atlantic Hurricane Forecast Taken Up a Notch
Current conditions in the tropics warrant marked revision in potential storm season scenarios. Read More
Sunset on Florida potato field day
Citrus
July 19, 2017
Researchers On a Mission to Find More Places for Growing Produce
Federal grant to aid exploration of food security solutions for the future. Read More
Citrus
July 19, 2017
Farm Labor Stories Making the News This Week
The agricultural labor shortage is strong enough that the consumer press is beginning to report on it regularly. Here are the stories making headlines this month. Read More
farm hacks collage
Citrus
July 19, 2017
Florida Grower Magazine is Seeking Your Farm Hacks
Life hacks are common in social media threads these days. They are those clever ideas or tricks aimed at making Read More
Rain drops on leaf
Citrus
July 14, 2017
Everglades Agricultural Area Farmers Winning at Water Quality
Annual report shows use of best management practices results in another massive reduction in phosphorus flow. Read More
Citrus
July 13, 2017
The Road is Long to Farm Bill 2018 [Opinion]
Participation in this process will be crucial to ensure your needs are understood and addressed. Read More
2015 FFVA Annual Convention crowd
Citrus
July 13, 2017
Trade Talk to Top Florida Fruit & Vegetable Association Convention Agenda
Trade issues are top of mind these days for specialty crop producers. Efforts have been underway since early this year Read More
Citrus
July 12, 2017
Shaky Florida Citrus Season Skids to a Stop
Final USDA tally confirms continuing downward trend of production in the HLB era. Read More
Smaller John Deere tractor for use in citrus screenhouse
Citrus Achievement Award
July 12, 2017
Encourage New Citrus Growth by Getting Back to Basics
2017 Florida Grower Citrus Achievement Award winner Ed Pines says producing crops under protective screen is a way to farm more and stress less. Read More
Beet-armyworms-on-a-tomato-plant
Citrus
July 12, 2017
Tomato Pests Can Be Induced to Cannibalism, New Study Shows
The University of Wisconsin's John Orrock says when beet armyworms are exposed to concentrations of methyl jasmonate, they will abandon eating tomatoes — and start eating one another. Read More
Citrus
July 12, 2017
USDA Pulls 8 Products from Approved Organic Production List
After a few months of speculation, the USDA's Agricultural Marketing Service has published its Sunset 2017 final rule on approved products for organic production and handling. Read More
Drone-aided photo of Ed Pines' CUPS
Varieties & Rootstocks
July 11, 2017
Florida Citrus Growers Going Inside to Think Outside the Box
Producing fruit under protective screen is developing into a viable option for sustaining the Sunshine State’s signature crop. Read More
Carl and Dustin Grooms of Fancy Farms
Business Planning
July 11, 2017
Young Florida Farmers Ready to Take the Reins
As growers age, the next generation is stepping up and stepping into leadership roles on the farm. Read More
The Latest
Citrus
July 26, 2017
Despite Controversy, Most Major Ag Group…
Most of the major agricultural associations are coming out in support of President Donald Trump’s nomination of Sam Clovis to Read More
Citrus
July 26, 2017
If We Want American Farm Workers, We Hav…
Now is the time to lobby for ways to attract American labor. One idea? Offer college tuition and student loan forgiveness in exchange for farm work. Read More
Citrus
July 23, 2017
USDA Invests $7.6 Million toward Benefic…
Projects to promote beneficial organisms as part of a pest control strategy. Read More
Citrus
July 22, 2017
Representative from Washington Proposes …
Move broadens use of H-2A to all of agriculture to include those with multiple crops and harvests. Read More
Citrus
July 20, 2017
Atlantic Hurricane Forecast Taken Up a N…
Current conditions in the tropics warrant marked revision in potential storm season scenarios. Read More
Citrus
July 19, 2017
Researchers On a Mission to Find More Pl…
Federal grant to aid exploration of food security solutions for the future. Read More
Citrus
July 19, 2017
Farm Labor Stories Making the News This …
The agricultural labor shortage is strong enough that the consumer press is beginning to report on it regularly. Here are the stories making headlines this month. Read More
Citrus
July 19, 2017
Florida Grower Magazine is Seeking Your …
Life hacks are common in social media threads these days. They are those clever ideas or tricks aimed at making Read More
Citrus
July 14, 2017
Everglades Agricultural Area Farmers Win…
Annual report shows use of best management practices results in another massive reduction in phosphorus flow. Read More
Citrus
July 13, 2017
The Road is Long to Farm Bill 2018 [Opin…
Participation in this process will be crucial to ensure your needs are understood and addressed. Read More
Citrus
July 13, 2017
Trade Talk to Top Florida Fruit & Ve…
Trade issues are top of mind these days for specialty crop producers. Efforts have been underway since early this year Read More
Citrus
July 12, 2017
Shaky Florida Citrus Season Skids to a S…
Final USDA tally confirms continuing downward trend of production in the HLB era. Read More
Citrus
July 12, 2017
Tomato Pests Can Be Induced to Cannibali…
The University of Wisconsin's John Orrock says when beet armyworms are exposed to concentrations of methyl jasmonate, they will abandon eating tomatoes — and start eating one another. Read More
Citrus
July 12, 2017
USDA Pulls 8 Products from Approved Orga…
After a few months of speculation, the USDA's Agricultural Marketing Service has published its Sunset 2017 final rule on approved products for organic production and handling. Read More
Business Planning
July 11, 2017
Young Florida Farmers Ready to Take the …
As growers age, the next generation is stepping up and stepping into leadership roles on the farm. Read More
Citrus
July 10, 2017
Florida Reveals Its Latest Woman of the …
2017 award recipient a true trailblazer and champion for the advancement of the state’s farming industry. Read More
Citrus
July 10, 2017
Practical Solutions Are Bringing Precisi…
Growing the budding ag-tech sector to maturity likely will require a deeper meeting of the minds between technologists and agriculturists. Read More
Business Planning
July 10, 2017
Farming for Online Sales Will Require Gr…
Modern agriculture might not be for everyone, but it’s here to stay. Embrace it. Read More